The International GCE Webinar is a monthly webinar series, open to all, featuring talks on cutting-edge applications of genetic code expansion (GCE) technologies as well as advances in the GCE field. It is organized by the Oregon State University GCE4All Research Center and hosted by Rick Cooley and John Lueck. The webinar is meant to be of value to both developers and users of GCE, as well as anyone interested in learning more about it. Speakers will be from around the globe, and we invite speaker and topic suggestions.
The webinars run from October through June and occur on the third Thursday of each month at 10 a.m. Eastern Time (corresponding to 2 p.m. GMT March-October and 3 p.m. GMT November through February). The general format is to give each of the two speakers 30 minutes which we recommend be divided into a presentation of 20-25 minutes followed by 5-10 minutes of discussion.
When possible, seminars will be recorded and made available for viewing on the GCE4All YouTube Channel; as this will not be possible for all seminars, please make it a priority to register for and participate in the live events. Although the seminars are free and open to all, pre-registration is required to get the specific link for each seminar.
December 15th, 2022
Investigations of multi-domain DNA/RNA binding protein machines using FEncAA
Edwin Antony (Saint Louis University, School of Medicine)
Our research focuses on DNA and RNA binding proteins that possess disparate nucleic acid and protein-protein interaction domains that are connected by disordered linkers. The inherent flexibility in the architecture enables formation of a wide array of configurations depending on the context of the DNA/RNA. To investigate how individual domains function to impart functional specificity, our group utilizes fluorescence-enhancement through non-canonical amino acids (FEncAA) to capture the dynamics, on-off rates, diffusion, and remodeling. Studies on Replication Protein A will be presented as a example of this approach.
Decoding the Language of Aromatic Side-Chains in Ion Channels and Receptors
Chris Ahern (University of Iowa)
January 19th, 2023
Alanna Schepartz (University of California, Berkeley)
Parameter Optimization for Efficient Orthogonal Translation: Enzyme Specificity & Solubility, Metabolic Availability & Stability of Amino Acid Analogs
Nediljko Budisa (University of Manitoba, Canada)
February 16th, 2023
Engineering Biomaterials Bearing Non-Canonical Amino Acids
Jin Kim Montclare (New York University)
Using GCE and Bioorthogonal Click Chemistry for Protein Labeling (Focus on Microscopy & Neurology Applications)
Ivana Nikic-Spiegel (Tuebingen)
March 16th, 2023
How to use Genetically Encoded Photo Cross-linking Amino Acids to Define Binding Sites of Peptide Toxins and Neuropeptides on Ligand-gated Ion Channels
Stephan Pless (University of Copenhagen)
GCE and PTMs and our work on Ubiquitylation
Kathrin Lang (ETH Zurich)
April 20th, 2023
May 18th, 2023
Sharona Gordon (University of Washington)
Fluorescent Amino Acid Method Development
James Petersson (University of Pennsylvania)
June 15th, 2023
F-NMR with Fluorinated Amino Acids
Angela Gronenborn (University of Pittsburgh)
October 20th, 2022
A Journey from In Vitro Misacylated tRNAs to Yeast Synthetic Biology
Virginia Cornish (Columbia University)
Rick Cooley (Oregon State University)
November 17th, 2022
Irene Coin (Leipzig)
Anthony Green (Manchester, UK)
GCE Community Networking
A resource available for more extensive discussion and networking among those learning about, using and/or developing GCE technologies is the GCE bulletin board – GCEbb – a forum for email-based discussions. More information about the GCEbb, including how to join the email list, can be found at the GCEbb home page. We are looking forward to having you join our GCE conversations!