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International GCE Webinar

Group photo of attendees of the 2018 GCE Conference held at OSU. The group is standing outside in front of the conference building.

The International GCE Webinar is a monthly webinar series, open to all, featuring talks on cutting-edge applications of genetic code expansion (GCE) technologies as well as advances in the GCE field. It is organized by the Oregon State University GCE4All Research Center and hosted by Rick Cooley and John Lueck. The webinar is meant to be of value to both developers and users of GCE, as well as anyone interested in learning more about it. Speakers will be from around the globe, and we invite speaker and topic suggestions.

Webinar mechanics

The webinars run from October through June and occur on the third Thursday of each month at 10 a.m. Eastern Time (corresponding to 2 p.m. GMT March-October and 3 p.m. GMT November through February). The general format is to give each of the two speakers 28 minutes which we recommend be divided into a presentation of 18-23 minutes followed by 5-10 minutes of discussion.

Registration

When possible, seminars will be recorded and made available for viewing on the GCE4All YouTube Channel; as this will not be possible for all seminars, please make it a priority to register for and participate in the live events. Although the seminars are free and open to all, pre-registration is required to get the specific link for each seminar.

Register here 
Check out our YouTube Channel 

Upcoming Webinars

March 21th, 2024

Genetically Encoded Bioorthogonal Chemistry in Living Systems

Peng Chen (Peking University, China)

Employing small molecules or other chemical means to modulate the function of an intracellular protein of interest, particularly in a gain-of-function fashion, remains highly desired but challenging. In this talk, I will introduce a “genetically encoded chemical decaging” strategy that relies on our recently developed bioorthogonal cleavage reactions to control protein activation in living systems. These reactions exhibit high efficiency and low toxicity for decaging the chemically “masked” lysine or tyrosine residues on intracellular proteins, allowing the gain-of-function study of individual enzymes within living cells and mice. Most recently, with the assistance of computer-based design and screening, we further expanded our method from “precise decaging” of enzyme active-sites to “proximal decaging” of enzyme pockets. This new method, termed Computationally Aided and Genetically Encoded Proximal Decaging” (CAGE-prox) (CAGE-prox), showed general applicability for switching on the activity of a broad range of proteins under living conditions. I will end by showcasing exciting applications of our CAGE-prox technique on: i) constructing orthogonal and mutually exclusive kinase signaling cascades; ii) temporal caspase activation for time-resolved profiling of proteolytic events upon apoptosis; and iii) on-demand activation of bacterial effectors as potential protein prodrugs for cancer therapy. Finally, by coupling with the proximity-labeling enzymes that have been used for subcellular targeting, we further developed a spatial-temporal resolved proteomics strategy for subcellular proteome profiling in living cells.

Sarah Lovelock (The University of Manchester, UK)

April 18th, 2024

Birgit Wiltschi (University Vienna, Austria)

Abhishek Chatterjee (Boston University, USA)

May 16th, 2024

Barbara Mueller (University of Heidelberg, Germany)

Alanna Schepartz (UC Berkeley, USA)

June 20th, 2024

Katrin Stuber (University of Copenhagen, Denmark)

Simon Kienle (University of Konstanz, Germany)

Past Webinars

October 19th, 2023

Expanding the non-canonical amino acid toolbox for mammalian cells (Recording)

Simon Elsässer (Karolinska Institutet, Sweden)


Sugars and Proteins

Ben Davis (University of Oxford, UK)

November 16th, 2023

Utilizing GCE to Probe Noncovalent Interactions Driving Histone PTM Recognition

Chris Travis (UNC, USA)


The cX-Zyme: A Halogen Bond Catalyzed DNA Nuclease (Recording)

P. Shing Ho (Colorado State University, USA)

December 2023

No webinar due to holidays

January 2024

Engineering post-translationally modified selenoproteins in E. coli (Recording)

Natalie Krahn (University of Georgia, USA)


Chemically expanded antibody engineering on the yeast surface: covalent antibodies and more (Recording)

Jim van Deventer (Tuffs University, USA)

February 15th, 2024

Applications of Genetic Code Expansion and Bioorthogonal Chemistry to Study Membrane Proteins (Recording)

Tom Sakmar (The Rockefeller University, USA)


Novel molecular insights into the Orai1 channel dynamics using photocrosslinking (Recording)

Isabella Derler (University Linz, Austria)

October 20th, 2022

A Journey from In Vitro Misacylated tRNAs to Yeast Synthetic Biology

Virginia Cornish (Columbia University)


PermaPhos: Revealing New Functions of Phosphorylated Proteins (Recording)

Rick Cooley (Oregon State University)

November 17th, 2022

Genetically encoded chemical tools for protein studies in the live cell (Recording)

Irene Coin (Leipzig)


Design and Evolution of Enzymes with Non-Canonical Catalytic Mechanisms (Recording)

Anthony Green (Manchester, UK)

December 15th, 2022

Investigations of multi-domain DNA/RNA binding protein machines using FEncAA (Recording)

Edwin Antony (Saint Louis University, School of Medicine)


Decoding the Language of Aromatic Side-Chains in Ion Channels and Receptors (Recording)

Chris Ahern (University of Iowa)

January 19th, 2023

How to make a thermostable ribosome

Jamie H. D. Cate (University of California, Berkeley)


Methodological challenges of an expanded genetic code (Recording)

Nediljko Budisa (University of Manitoba, Canada)

February 16th, 2023

Engineering Biomaterials Bearing Non-Canonical Amino Acids (Recording)

Jin Kim Montclare (New York University)


Expanded genetic code for live-cell fluorescent protein labeling in (neuro)biology (Recording)

Ivana Nikic-Spiegel (Tuebingen)

March 16th, 2023

Identification of neuropeptide and toxin binding sites on a membrane-embedded receptor through crosslinking (Recording)

Stephan Pless (University of Copenhagen)


Expanding the genetic code – new chemistries for biology

Kathrin Lang (ETH Zurich)

April 20th, 2023

A mutagenesis toolkit for bromodomain interactions

Andrea Cochran (Genentech, Inc.)


Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression (Recording)

Jeffery M. Tharp (Indiana University School of Medicine)

May 18th, 2023

Protein chemistry: an unnatural approach to unravelling the mystery of allostery (Recording)

Sharona Gordon (University of Washington)


Applications of the Fluorescent Amino Acid Acridonylalanine (Recording)

James Petersson (University of Pennsylvania)

June 15th, 2023

Exploiting Fluorine for In-cell NMR

Angela Gronenborn (University of Pittsburgh)


NMR isotope-labelled, serine-16 phosphorylated, amelogenin – the key to brighter smiles and understanding biomineralization (Recording)

Garry Buchko (PNNL)

GCE Community Networking

A resource available for more extensive discussion and networking among those learning about, using and/or developing GCE technologies is the GCE bulletin board – GCEbb ­– a forum for email-based discussions. More information about the GCEbb, including how to join the email list, can be found at the GCEbb home page. We are looking forward to having you join our GCE conversations!

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