The International GCE Webinar is a monthly webinar series, open to all, featuring talks on cutting-edge applications of genetic code expansion (GCE) technologies as well as advances in the GCE field. It is organized by the Oregon State University GCE4All Research Center and hosted by Rick Cooley and John Lueck. The webinar is meant to be of value to both developers and users of GCE, as well as anyone interested in learning more about it. Speakers will be from around the globe, and we invite speaker and topic suggestions.
International GCE Webinar
Webinar mechanics
The webinars run from October through June and occur on the third Thursday of each month at 10 a.m. Eastern Time (corresponding to 2 p.m. GMT March-October and 3 p.m. GMT November through February). The general format is to give each of the two speakers 28 minutes which we recommend be divided into a presentation of 18-23 minutes followed by 5-10 minutes of discussion.
Registration
When possible, seminars will be recorded and made available for viewing on the GCE4All YouTube Channel; as this will not be possible for all seminars, please make it a priority to register for and participate in the live events. Although the seminars are free and open to all, pre-registration is required to get the specific link for each seminar.
Register here
Check out our YouTube Channel
Upcoming Webinars
April 18th, 2024
Non-canonical amino acids as building blocks for recombinant proteins and peptides
Birgit Wiltschi (University Vienna, Austria)
Non-canonical amino acids carry unusual side chains such that their translation into a target polypeptide sequence can provoke structural, chemical, or functional modifications normally not found in nature. We have used non-canonical amino acids to leverage the potential of lectins and small antigen-binding proteins for targeted cancer cell killing and drug delivery. While the engineering of proteins with non-canonical amino acids has gained increasing popularity in the academic environment, it faces specific challenges upon upscaling. I will highlight the challenges of the method in industrial biotechnology and discuss possible approaches to meet them.
A mammalian cell-based directed evolution platform to enhance the efficiency of ncAA incorporation systems
Abhishek Chatterjee (Boston University, USA)
Controlled incorporation of non-canonical amino acids (ncAAs) into proteins in living cells has emerged as a powerful tool for biological research and biotechnology. The Chatterjee group focuses on advancing the scope of this technology for both probing complex biological questions, as well as to create opportunities to develop next-generation biotherapeutics. Our research goals include: 1) Creating new platforms to genetically encode ncAAs with diverse chemical structures, 2) Developing new bioorthogonal conjugation reactions that are compatible with existing bioconjugation chemistries for precise protein labeling with multiple distinct entities, 3) Creating next-generation biotherapeutics, such as precisely modified therapeutic proteins and viral vectors for gene therapy, 4) Investigating the roles of various post-translational modifications of human proteins, and 5) Analyzing dynamic changes in the cellular proteome. This presentation will focus on the development of a mammalian cell-based directed evolution platform to enhance the efficiency of ncAA incorporation systems in these cells
May 16th, 2024
Barbara Mueller (University of Heidelberg, Germany)
Alanna Schepartz (UC Berkeley, USA)
June 20th, 2024
Katrin Stuber (University of Copenhagen, Denmark)
Simon Kienle (University of Konstanz, Germany)
2023-2024 Webinar Recordings
October 19th, 2023
Expanding the non-canonical amino acid toolbox for mammalian cells
Simon Elsässer (Karolinska Institutet, Sweden)
November 16th, 2023
The cX-Zyme: A Halogen Bond Catalyzed DNA Nuclease
P. Shing Ho (Colorado State University, USA)
January 18th, 2024
Engineering post-translationally modified selenoproteins in E. coli
Natalie Krahn (University of Georgia, USA)
Chemically expanded antibody engineering on the yeast surface: covalent antibodies and more
Jim van Deventer (Tuffs University, USA)
February 15th, 2024
Applications of Genetic Code Expansion and Bioorthogonal Chemistry to Study Membrane Proteins
Tom Sakmar (The Rockefeller University, USA)
Novel molecular insights into the Orai1 channel dynamics using photocrosslinking
Isabella Derler (University Linz, Austria)
March 21th, 2024
Genetically Encoded Bioorthogonal Chemistry in Living Systems
Peng Chen (Peking University, China)
2022-2023 Webinar Recordings
October 20th, 2022
PermaPhos: Revealing New Functions of Phosphorylated Proteins
Rick Cooley (Oregon State University)
November 17th, 2022
Genetically encoded chemical tools for protein studies in the live cell
Irene Coin (Leipzig)
Design and Evolution of Enzymes with Non-Canonical Catalytic Mechanisms
Anthony Green (Manchester, UK)
December 15th, 2022
Investigations of multi-domain DNA/RNA binding protein machines using FEncAA
Edwin Antony (Saint Louis University, School of Medicine)
Decoding the Language of Aromatic Side-Chains in Ion Channels and Receptors
Chris Ahern (University of Iowa)
January 19th, 2023
Methodological challenges of an expanded genetic code
Nediljko Budisa (University of Manitoba, Canada)
February 16th, 2023
Engineering Biomaterials Bearing Non-Canonical Amino Acids
Jin Kim Montclare (New York University)
Expanded genetic code for live-cell fluorescent protein labeling in (neuro)biology
Ivana Nikic-Spiegel (Tuebingen)
March 16th, 2023
Identification of neuropeptide and toxin binding sites on a membrane-embedded receptor through crosslinking
Stephan Pless (University of Copenhagen)
April 20th, 2023
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression
Jeffery M. Tharp (Indiana University School of Medicine)
May 18th, 2023
Protein chemistry: an unnatural approach to unravelling the mystery of allostery
Sharona Gordon (University of Washington)
Applications of the Fluorescent Amino Acid Acridonylalanine
James Petersson (University of Pennsylvania)
June 15th, 2023
NMR isotope-labelled, serine-16 phosphorylated, amelogenin – the key to brighter smiles and understanding biomineralization (Recording)
Garry Buchko (PNNL)
GCE Community Networking
A resource available for more extensive discussion and networking among those learning about, using and/or developing GCE technologies is the GCE bulletin board – GCEbb – a forum for email-based discussions. More information about the GCEbb, including how to join the email list, can be found at the GCEbb home page. We are looking forward to having you join our GCE conversations!